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doi: 10.1046/j.1365-2826.1999.00330.x 42 KamegaiJ.WakabayashiI.MiyamotoK.UntermanT

An Afe I restriction site directly upstream of the Strep -tag II, together with the unique Xba I restriction site in front of the whole reading frame, was subsequently used to exchange the entire coding region for sfGFP by the ones for different other POIs (see Supplementary Table S1)

Real time polymerase chain reaction (RT-PCR) Following homogenization of the skin tissues (n = 3), total ribonucleic acid (RNA) was extracted using the trizol technique in accordance with the manufacturers instructions

VAN DIJK, L.P

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